Nano Biomedicine
ORIGINAL ARTICLE

Montipora digitata Exoskeleton Derived Aragonite Particles are Useful Scaffold for Tissue-engineered Vascular Graft in Vitro

Tomoharu OKAMURA1, Naoya UEMURA2, Shunsuke BABA2, Naoko YASUDA3, Hideyuki YAMASHIRO3, Koichi IMAI4, Tetsunari NISHIKAWA5, Hideo SHIMIZU6, Muneyasu SHIDA7, Kazuya TOMINAGA1, and Akio TANAKA8

 1Department of Oral Pathology, 2Department of Implantology,
Osaka Dental University, Osaka, Japan, 3Biology and Marine Science,
Faculty of Science University of the Ryukyu, Okinawa, Japan
4Department of Biomaterials, 5Innovations in Dental Education,
Osaka Dental University, Osaka, Japan, 6Department of Clinical Gene Therapy,
Graduate School of Medicine, Osaka University, Osaka, Japan
7Department of Endodontics, 8Department of Pathology,
Osaka Dental University, Osaka, Japan
Nano Biomed 2017; 9(2): 105-111, (Dec 30, Nano Biomedicine)
Synopsis
Designing a preoperative tissue engineering vascular graft (TEVG) that is immunologically compatible with the host is one of the goals of vascular tissue engineering. Angiogenesis is an important early step in wound healing, and the three-dimensional formation of capillaries using scaffold contributes greatly to the success of bone regeneration therapy. In this study, aragonite particles derived from exoskeleton of Montipora digitata (a kind of hard coral) as a scaffold of TEVG were applied to three-dimensional culture of normal human dermal skin fibroblasts and normal human umbilical cord vein endothelial cells, and the effect was observed morphologically. As a result, the particles promoted cell proliferation and capillary differentiation three-dimensionally in vitro. Also, incorporation of calcium from the aragonite particles into co-cultured normal human dermal skin fibroblasts and normal human umbilical vein endothelial cells was observed. Thus, aragonite particles were inferred to be a useful scaffold for 3 dimensional capillary culture.

Key words: Coral exoskeleton derived aragonite, Bone substitute material, Porous material, Angiogenesis, Tissue engineering

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