Nano Biomedicine
ORIGINAL ARTICLE

Study of ES Cell Differentiation using Three-dimensional Culture with Silica Fiber

Koichi IMAI1, Shunji KUMABE2, Yoshiaki ONO3, Hidenori MATSUMOTO4, and Tetsunari NISHIKAWA4

1Department of Biomaterials, 2Department of Oral Anatomy,
3Department of Geriatric Dentistry, 4Department of Innovation in Dental Education,
Osaka Dental University, Osaka, Japan
Nano Biomed 2017; 9(2): 55-60, (Dec 30, Nano Biomedicine)
Synopsis
Usability of high-purity silica fibers as a 3-dimensional carrier of ES cell differentiation-inducing culture has not yet been reported. CellBed® has been reported to have high chemical stability and superior affinity to cells as a 3-dimensional culture carrier. In addition, cells are not detached during medium change, being another characteristic. Accordingly, there may be no interference with ES cell proliferation. We have reported no interference of various types of collagen with ES cell differentiation. However, the influence of high-purity silica fibers on normal ES cell differen-tiation as a 3-dimensional culture carrier is unclear.
In this study, the cell proliferation and differentiation levels of ES-D3 cells cultured in Cell-Bed® were measured and these were comparable with those using pig-derived collagen gel and fish (Tilapia)-derived collagen sponge. The influence of actual biological materials and drugs on ES cell differentiation may be evaluated using CellBed®.

Key words: Silica Fiber, ES Cell, Differentiation, 3D, Tilapia

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