Nano Biomedicine

Inference of Embryotoxicity of Titanium Alloy Composition Metal Chemicals as an Oral Implant Body with EST Protocol

Koichi IMAI1, Tsubasa SHIRAI1, Hiroki ISHIKAWA2, Tamaki NAKANISHI2, and Shosuke MORITA2

1Department of Biomaterials, 2First Department of Oral and Maxillofacial Surgery,
Osaka Dental University, Osaka, Japan

Nano Biomed 2017;9(1):9-14, (Jun 30, Nano Biomedicine)

The risks of embryotoxicity of pure Ti and Ti alloys used as oral implant materials have not been sufficiently elucidated. The embryonic stem cell test (EST), which allows screening of the fetal teratogenicity of chemical substances, has already been demonstrated to show high predictability by international validation, mainly in Europe. We evaluated the embryotoxicity levels of constituent element ions (Ti, V, Al, Nb, Ta, and Zr) of implants using the EST. As a result, the V ion was found to be associated with some embryotoxicity risks, while the other metal ions showed no embryotoxicity. As previously demonstrated, the V ion shows marked cytotoxicity and carcinogenicity. Therefore, Ti alloys without V ions, such as Ti-6Al-7Nb, should be widely used as implant materials.

Key words: embryotoxicity, Ti, Ti alloy, EST, in vitro

"Nano Biomedicine" Homepage(English)

"Nano Biomedicine" Homepage(Japanese)