Nano Biomedicine

Effects of Addition of Nano-hydroxyapatite to Highly-pressed Collagen on Osteogenic Differentiation in Osteoblastic SaOS-2 Cells

Koji IKEDA1, Masayuki TAIRA2, Jun YOKOTA1, Masayuki HATTORI2, Akira ISHISAKI3, and Hisatomo KONDO1

1Department of Prosthodontics and Oral Implantology,
Iwate Medical University School of Dentistry, Iwate, Japan.
2Department of Biomedical Engineering; Iwate Medical University and
3Division of Cellular Biosignal Sciences, Department of Biochemistry,
Iwate Medical University, Iwate, Japan

Nano Biomed 2016;8(2):91-100, (Dec 30, Nano Biomedicine)

We produced highly pressed nano-hydroxyapatite/collagen composites (P-nHAP/COL) by Newton press. The aim of this study was to investigate osteogenic differentiation profiles in osteoblastic SaOS-2 cells cultured on P-nHAP/COL and pressed collagen (P-COL) as control for 1, 2, 3 and 4 weeks. Total RNA were extracted from cultured cells, and expressions of osteogenic-differentiation related genes were evaluated with quantitative real-time (RT) PCR using primer sets of alkaline phosphatase (ALP), type 1 collagen (COL1), bone Sialo protein (BSP) and osteocalcin precursor (BGLAP) genes as well as β-actin gene. The cultured cells were also observed with scanning electron microscopy (SEM). RT-PCR analyses revealed that osteogenic differentiation was more accel-erated on P-nHAP/COL than on P-COL, especially revealed by up-regulation with time of BGLAP gene. In SEM images, SaOS-2 cells on P-COL were either cubic or fibroblastic up to 4 weeks, but those on P-nHAP/COL appeared as granular or highly stretched. The obtained results suggested that P-nHAP/COL could be employed as a new osteo-conductive bone substitute material.

Key words: Osteoblastic SaOS-2 cells, Nano-hydroxyapatite, Collagen composite, Newton press, Osteogenic differentiation

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