Nano Biomedicine

Modification of Composite Resin Surface with Carbon Nanotubes Enhances Cell Proliferation

Saori TANAKA1,2 , Hirofumi MIYAJI2, Erika NISHIDA2, Kana INOUE2, Saori MIYATA2, Akihito KATO2, Izumi KANAYAMA2, Shusuke MURAKAMI2, Kohei KAWAMOTO2, Bunshi FUGETSU3, Toru TANAKA4, Hiroko TAKITA5, Tadashi IIZUKA5, and Masamitsu KAWANAMI2
1Division of General Dentistry Center for Dental Clinics,
2Department of Periodontology and Endodontology, Hokkaido University
Graduate School of Dental Medicine, Sapporo, Japan
3Nano-Agri Lab, Policy Alternatives Research Institute,
The University of Tokyo, Tokyo, Japan
4Department of Restorative Dentistry,
5Support Section for Education and Research, Hokkaido University
Graduate School of Dental Medicine, Sapporo, Japan

Nano Biomed 2016;8(1): 27-34, (June 30, Nano Biomedicine)

Composite resin (CR) is used in vivo as a root perforation sealer and as a retrograde obturation material following apicoectomy, but it does not possess adequate biocompatibility. Effective biological modification of the CR surface is thus needed to improve its biocompatibility. Carbon nanotubes (CNTs) are a type of nanocarbon material formed from cylindrical graphene sheets with a diameter of about 10 to 200 nm. CNTs have garnered attention for useful properties such as promoting cell adhesion and proliferation. The present study evaluated cell adhesion and proliferation on CR discs coated with CNTs. CR discs were coated by immersion in a dispersion of CNTs (Nanocyl NC7000, 9.5 nm diameter). The CNT coating was then observed with a scanning electron microscope (SEM) to investigate its surface properties. The effects of CNT coating were also investigated by conducting cell culture assay using osteoblasts. The coating process enabled CNTs to adhere to the resin surface, resulting in good adhesion and spreading of the osteoblasts that indicated a high level of cell proliferation. The study findings showed that coating the surface of CRs with CNTs improved their biocompatibility.

Key words: Carbon nanotubes, Composite Resin, Cell proliferation, Biocompatibility

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