Nano Biomedicine
ORIGINAL ARTICLE

Comparison of the Bone Forming Ability of Different Sized-alpha Tricalcium Phosphate Granules using a Critical Size Defect Model of the Mouse Calvaria

 Tomoko TOKUDA1, Yoshitomo HONDA2, Yoshiya HASHIMOTO3, and Naoyuki MATSUMOTO4
1Graduate School of Dentistry (Department of Orthodontics),
2Institute of Dental Research, 3Department of Biomaterials,
4Department of Orthodontics, Osaka Dental University, Osaka, Japan
Nano Biomed 2015;7(2): 63-71, (Dec 30, Nano Biomedicine)
Synopsis
Alpha-tricalcium phosphate (α-TCP) has been investigated extensively as an artificial bone graft; however, the relationship between size of α-TCP particles and its bone-formation capability is not clear. In the present study, we compared the bone-formation capability of two different sized porous α-TCP particles (α-TCP200 [under 200 μm ], and α-TCP600 [500-600 μm]) in critical-sized bone defects in mouse calvaria up to 12 wk after implantation. Scanning electron microscopy revealed that both particles possessed similar smooth surface with porous structure. Before implantation, inter-particle size and specific surface area of α-TCP200 were 27 mm and 0.40 m2/g and of α-TCP600 were 209 mm and 0.24 m2/g, respectively. Histomorphometric analysis after implantation of the particles revealed that both particles promoted osteoconduction. At 12 wk, α-TCP200 induced superior bone formation than α-TCP600. At 4 wk, α-TCP200 showed more hydrolysis than α-TCP600. These results indicate that the size of α-TCP particles may influence their bone-formation capability in critical-sized bone defects of mouse calvaria. This effect might be partially due to the difference in the hydrolysis speed of different-sized particles.

Key words: alpha tricalcium phosphate, bone formation, particle, calvaria, hydrolysis

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