Nano Biomedicine

Preliminary Experimental Study by the Cell Differentiation and a Cell Viability on the Cell Culture Environment with the Linear Structures of Nylon Fiber Bundles

Koichi IMAI1, Tsubasa SHIRAI2, Hiroki ISHIKAWA3, Yoshitomo HONDA4, Muneyasu SHIDA5, Fumiya OGAWA6, Hirofumi SAWAI7, Tadashi OHKUBO7, Tatsuro MIYAKE8, and Fumio WATARI9
1Department of Biomaterials, 2Graduate School of Dental Medicine (Biomaterials),
3Graduate School of Dental Medicine (First Dept. of Oral and Maxillofacial Surgery),
4Institute of Dental Research, 5Department of Endodontics,
6Department of Postgraduate Clinical Training,
7Department of Internal Medicine, Osaka Dental University, Osaka, Japan
8Department of Oral Health Sciences, Faculty of Nursing and Health Care, Baika Women's University, Osaka, Japan
9Emeritus professor of Hokkaido University, Sapporo, Japan

Nano Biomed 2015;7(1): 5-10, (Jun 30)

The roles of surface configurations of biomaterials in the regeneration of surrounding tissues should be considered for the application of biomaterials to regenerative medicine. Many reports have been published on the relationship between the surface configurations of oral implants and osseointegration.
To obtain basic data on the effects of the surface configurations of biomaterials, we examined the effects of the linear structures of nylon fiber bundles with a diameter of about 10 m on cell differentiation and those of two types of monomer and metal ion exposure on cytotoxicity levels.
Cell differentiation was examined using alkaline phosphatase activities, demonstrating that absorbance was slightly decreased on dish bottoms, compared with linear structures, with MC3T3-E1 and C2C12 cells, but remained unchanged with ES-D3 cells. Cytotoxicity levels were examined based on cell viability using the MTT method. The linear structures tended to decrease the viability of MC-3T3-E1 and C2C12 cells, as compared with the dish bottoms. On the other hand, no difference was noted in the viability of ES-D3 cells. Microscopically, elongated fibrous structures were observed along the striatum structures in MC-3T3-E1 and C2C12 cells, while no marked morphological difference was noted in ES-D3 cells.
In the present study, linear structures showed different differentiation and cytotoxicity levels between MC3T3-E1 and C2C12 cells. Unlike cells on dish bottoms, most cells surrounding biomaterials in vivo cannot extend without limitation in multiple directions. Thus, in vitro cell differentiation and cytotoxicity levels may vary with the surface configurations of biomaterials.

Key words: surface configuration, biomaterials, cell differentiation, cytotoxicity

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