Nano Biomedicine
ORIGINAL ARTICLE

Effects of C60 Fullerene on Cell Differentiation with EL-M3 and ES-R1-EGFP B2/EGFP Cell Lines

 Koichi IMAI1, Tetsunari NISHIKAWA2, Tomoharu OKAMURA2, Akio TANAKA2, Kazuhiko SUESE3,4, Yoshitomo HONDA5, Tsubasa SHIRAI6, Fumiya OGAWA7, Hirofumi SAWAI8 and Fumio WATARI9
 1Department of Biomaterials, Osaka Dental University, Osaka, Japan
2Department of Oral Pathology, Osaka Dental University, Osaka, Japan
3Department of Esthetic Dentistry, Osaka Dental University, Osaka, Japan
4Department of Postgraduate Clinical Training, Osaka Dental University, Osaka, Japan
5Institute of Dental Research, Osaka Dental University, Osaka, Japan
6Graduate School of Dental Medicine (Biomaterials), Osaka Dental University, Osaka, Japan
7Department of Postgraduate Clinical Training, Osaka Dental University, Osaka, Japan
8Department of Internal Medicine, Osaka Dental University, Osaka, Japan
9Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan
Nano Biomed 2014;6(2): 78-84, (Dec 30)
Synopsis
The effects of nanomaterials on human reproduction and development remain unclear. Thus, their embryotoxicity should be examined to ensure the biological safety of the next generation. In the present study, the effects of C60 fullerene on cell differentiation were investigated using EL-M3 and ES-R1-EGFP B2/EGFP cell lines that require feeder cells, instead of the ES-D3 cells used for the EST method, an in vitro embryotoxicity test. As a result, the effects of C60 fullerene on cell differentiation increased in a concentration-dependent manner for both cell lines, demonstrating the absence of severe developmental toxicity. The developmental toxicity of C60 fullerene should be investigated for applications to new drugs.

Key words: C60 fullerene, embryotoxicity, EL-M3 cell, ES-R1-EGFP B2/EGFP cells

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