Nano Biomedicine
ORIGINAL ARTICLE

Influence of MWCNTs to Myocardial Contraction Rhythms on Differentiation of ES-D3 Cells in Three-dimensional Culture

 Koichi IMAI1, Tsubasa SHIRAI1, Fumio WATARI2, Tsukasa AKASAKA2,
Tetsunari NISHIKAWA3, Tomoharu OKAMURA3, Akio TANAKA3, Kazuhiko SUESE4,
Fumiya OGAWA5, Yoshitomo HONDA6, Hirofumi SAWAI7, and Hiromasa TAKASHIMA8
1 Department of Biomaterials, Osaka Dental University, Osaka, Japan
2 Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan
3 Department of Oral Pathology, Osaka Dental University, Osaka, Japan
4 Department of Esthetic Dentistry, Osaka Dental University, Osaka, Japan
5 Department of Postgraduate Clinical Training, Osaka Dental University, Osaka, Japan
6 Institute of Dental Research, Osaka Dental University, Osaka, Japan
7 Department of Internal Medicine, Osaka Dental University, Osaka, Japan
8 Ina Research Inc., Nagano, Japan
Nano Biomed 2014;6(1): 27-34, (Jun 30)
Synopsis
The embryotoxicity of multi-walled carbon nanotubes (MWCNTs) has been investigated using the embryonic stem cell test (EST) protocol. Embryoid bodies (EBs) were generated with the hanging drop culture of ES-D3 cells, followed by one-week culture in three-dimensionally cultured collagen gel with MWCNTs. The contraction of myocardia that differentiated in teratomas could be microscopically observed. However, the myocardial contraction rhythms were examined by image analysis, because they markedly varied. Without MWCNTs, the contraction rhythms of the myocardia were constant. Some teratomas showed peristalsis, suggesting differentiation into the gastrointestinal tract. However, MWCNTs mixture had no effect on the peristaltic rhythms. Conductive MWCNTs that contaminate the scaffolds may have electrical effects. This phenomenon should be further investigated because biological problems are a concern regarding MWCNTs.

Key words: Myocardial contraction, ES cells, MWCNTs, teratoma, differatiation

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