Synopsis
Using a rotating wall vessel (RWV) bioreactor, which simulates a microgravity environment for cells, approximately 150 osteosarcoma MG63/collagen sponge composites (3 mmΦ x 3 mm) were three-dimensionally cultured simultaneously in a cylindrical vessel (250 mL) to produce the same number of cancer tissues of an equal size and quality. Then, these tissues were exposed to the anti-cancer drug doxorubicin, and dose dependence of the effect of this drug on the viability of the cells was examined compared with a conventional two-dimensional culture system. The dose-dependent effect on three-dimensional tissues was markedly different from the two-dimensional culture because of differences in the culture systems.
Key words: anticancer drug, cancer tissue, RWV bioreactor
