Nano Biomedicine
ORIGINAL ARTICLE

The Effect of Carbon Ion Beam Irradiation for Hypoxia-Mediated Invasion of Glioblastoma

 Kazumasa MINAMI1, Masahiko KOIZUMI2, Yoshinosuke HAMADA1, 3, 4, Seiji MORI1,
Naomasa KAWAGUCHI1, Masashi MANABE4, Hiromasa IMAIZUMI2, Kana NAKATANI2, and Nariaki MATSUURA1
 1Department of Functional Diagnostic Science,
2Department of Medical Physics and Engineering,
3Department of Health Economics and Management, Osaka University Graduate School of Medicine
4Department of Pediatric Dentistry, Osaka Dental University

Nano Biomed 2014;6(1): 1-11, (Jun 30)
Synopsis
Glioblastoma is one of the most fatal malignant tumors because of its invasiveness and radioresistance. Unfortunately photon irradiation and hypoxic condition are reported to enhance cancer cells' aggressiveness respectively. The purpose of this study is to clarify the effect of carbon ion beam on invasive potential of glioblastoma cells. Relative Biological Effectiveness of A172 cells measured by the dose for 1% survival fraction was 2.22. X-irradiation and hypoxic condition promoted invasion and migration of A172 and T98G cells respectively, while carbon ion beam suppressed both the two capabilities enhanced by hypoxia. GTP-RhoA (active form) was increased by 0.5 Gy of X-irradiation. On the other hand RhoA was inactivated by 1 and 4 Gy of carbon ion beam. Carbon ion beam irradiation suppressed glioblastoma cellsf invasion and migration via inactivation of RhoA. Furthermore carbon ion beam also inhibited these abilities enhanced by hypoxia.

Key words: Carbon ion beam, invasion, glioblastoma

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