Nano Biomedicine
ORIGINAL ARTICLE

Embryotoxicity of the Multi-walled Carbon Nanotubes (MWCNTs) using the Three-dimensional Culture of ES-D3 cells

 Koichi IMAI1, Fumio WATARI2, Kazuhiko SUESE3, Yoshitomo HONDA4, and Hiromasa TAKASHIMA5

 1Department of Biomaterials, Osaka Dental University, Osaka, Japan
2Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan
3Osaka Dental University, School of Dental Technician and Hygienist, Osaka, Japan
4Institute of Dental Research, Osaka Dental University, Osaka, Japan
5Ina Research Inc., Nagano, Japan
Nano Biomed 2013;5(1):44-49, (Jun 30)
Synopsis
To examine the embryotoxicity of the multi-walled carbon nanotubes (MWCNTs), the effects of three-dimensional culture with a collagen gel scaffold containing MWCNTs on the differentiation level of ES-D3 cells were examined and compared with those of conventional two-dimensional culture.
In the present study, the rate of contraction of the pulsating myocardial cells decreased as the MWCNTs concentration increased, with no contraction observed at >25 µg. The contraction of the pulsating myocardial cells in teratomas showed uniform and rhythmic movements in the control group and non-uniform and non-rhythmic movements in the 2.5-10-µg groups.
Thus, the non-rhythmic contraction of the pulsating myocardial cells, and not the rate of con-traction determined by the EST method, may serve as a novel cell differentiation indicator.

Key words: ES-D3 cells, 3D, MWCNTs, rhythmic contraction, non-rhythmic contraction

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