Nano Biomedicine
ORIGINAL ARTICLE

Crystallization and Preliminary X-ray Diffraction Analysis of a SCO7809 Protein from Streptomyces coelicolor A3(2)

 Parvin BEGUM1, Bunshi FUGETSU1, Tsukasa AKASAKA2, and Fumio WATARI2

 1Laboratory of Environmental Medical Chemistry, Graduate School of Environmental Science,
2Department of Dental Materials and Engineering, Graduate School of Dental Medicine,
Hokkaido University, Sapporo, Japan.
Nano Biomed 2013;5(1):39-43, (Jun 30)
Synopsis
SCO7809 is a TetR family transcriptional factor. SCO7809 protein from Streptomyces coelicolor A3(2) genome was overexpressed in Rhodococcus erythropolis, purified and crystallized using sitting drop vapor diffusion method. Crystals of selenomethionine-substituted SCO7809 diffract to 2.8 Å resolutions. The crystal belongs to space group C2221 with unit-cell parameters of a = 95.6, b = 115.1, c = 247.7 Å. This is the first crystallographic study of recombinant SCO7809 protein.

Key words: SCO7809 protein, Streptomyces coelicolor A3(2), crystallographic, recombinant protein

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