Nano Biomedicine
ORIGINAL ARTICLE

Evaluation of Bone Regeneration of Apatite Coating Poly-L-lactide Scaffold in Rat Calvarial Defects

 Kenichirou YASUI1, Yoshiya HASHIMOTO2, Shunsuke BABA3, Shigeki HONTSU4, and Naoyuki MATSUMOTO5

 1Graduate School of Dentistry (Orthodontics),
2Department of Biomaterials, Osaka Dental University, Osaka Japan.
3Department of Oral Implantology, Osaka Dental University, Osaka Japan.
4Department of Biomedical Enginnering, School of Biology-Oriented Science and Technology,
Kinki University, Wakayama, Japan
5Department of Orthodontics, Osaka Dental University, Osaka Japan
Nano Biomedicine 2012;4(2):133-142, (Dec 30)
Synopsis
This study examined in vivo biocompatibility of poly-L-lactide (PLLA) scaffolds coated with hydroxyl apatite (HA) by pulsed laser deposition (PLD). A thin HA film was made on PLLA discs by an ArF PLD operating at a repletion rate of 10 Hz. Cranial bone defects were created in rats and filled with non-HA-coated PLLA scaffold (PLLA) or HA-coated PLLA scaffold (HAPLLA). All specimens were evaluated micro-radiographically and with histological analysis at 2, 4, and 6 weeks. The presence of calcium and phosphorous on the surface as well as in the interior of the PLLA was confirmed by energy dispersive analysis. In vitro cell-spread tests showed that the human osteoblasts spread more on HAPLLA than on PLLA. Upon transplanting calvarial defects, the in vivo hard tissue responses suggested earlier restoration of the defects with HAPLLA than with PLLA.

Key words: bone tissue engineering, hydroxyl apatite, poly-L-lactic acid, pulsed laser deposition, rat calvarial defect

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