Nano Biomedicine
ORIGINAL ARTICLE

Quantitative Real-time RT-PCR Analyses of DNA-damage-recovery-related Gene Expressions of Mouse Macrophage-like Cell Line RAW264 when Exposed to IC50 Nickel Ions

Masayuki TAIRA1, Tadayoshi KAGIYA2, Minoru SASAKI3, and Shigenobu KIMURA3

1Department of Biomedical Engineering,
2Division of Functional Morphology, Department of Anatomy,
3Division of Molecular Microbiology, Department of Microbiology,
Iwate Medical University, Iwate, Japan
Nano Biomedicine 2011;3(2): 294-399, (Dec 30)
Synopsis
The primary purpose of this study was to analyze the effects of IC50 nickel (Ni) (2+) ions on expressions of seven DNA-damage-recovery genes (Mre11a, Dclre1a, Ddb1, Msh3, Rad51, Ercc1 and Xpa) of mouse macrophage-like cell line RAW264 cultured for 4h and 24h. Quantitative real-time RT-PCR analyses revealed that IC50 Ni (2+) ions significantly down-regulated expressions of these seven genes with time. Additional analyses clarified that IC50 Ni (2+) ions up-regulated expression of two cell-protection genes (Hmox1 and Ier3) and one anti-metal-toxicity gene (MT1) of RAW264 cells with time. Taken together, our data suggested that excessive (i.e. IC50 concentration) Ni (2+) ions overcame intrinsic cell protective mechanisms of RAW264 cells, and inhibited productions of DNA-damage-recovery genes, leading to reduced cell viabilities.

Key words: macrophage, nickel ions, LPS, gene expression, DNA damage <

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