Nano Biomedicine
ORIGINAL ARTICLE

An Attempt to Study of the C60 Fullerene on Differentiation of Mouse ES cells

Koichi IMAI1, Fumio WATARI2, Tetsunari NISHIKAWA3, Akio TANAKA3, Akito TANOUE4,
Kazuaki NAKAMURA4, and Hiromasa TAKASHIMA5

 1Department of Biomaterials, Osaka Dental University, Osaka, Japan
2Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan
3Department of Oral Pathology, Osaka Dental University, Osaka, Japan
4Department of Pharmacology, National Research Institute for Child Health and Development, Tokyo, Japan
5Hatano Research. Institute, FDSC, Kanagawa, Japan
Nano Biomedicine 2011;3(2): 288-293, (Dec 30)
Synopsis
We examined the differentiation capacity of mouse embryonic stem (ES) cells cultured on C60 fullerene, as well as cell viability effects, employing a cellular differentiation technique for the embryonic stem cell test (EST). The results demonstrated minor effects on differentiation assay, beating myocardial rate of the dispersed solution was noted in 72% of the control. In addition, there was no influence on cell viability. In this study, the C60 fullerene did not influence the differentiation of ES-D3 cells and "non embryotoxicity". We here investigated C60 fullerene dispersed in a medium. We expected a result of natural dispersion of C60 fullerene into the medium by agitation as possible. This is probably because C60 fullerene did not dispersed. Fullerene particles should be sufficiently dispersed in media. In the future, the biological safety should be comprehensively ex-amined by improving dispersion in medium.

Key words: C60 fullerene, embryotoxicity, ES cells<

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