Nano Biomedicine
ORIGINAL ARTICLE

Cell Culture on Nanopillar Sheets - Mesenchymal Stem Cells form Spheroids under Chondrogenic Differentiation Depending on Nanopillar Sizes -

Toshimasa UEMURA1, Sun-Jung KIM2, Kosuke KUWABARA3, and Akihiro MIYAUCHI3

1Nanosystem Research Institute (NRI), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan
2Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan
3Hitachi Research Laboratory, Hitachi Ltd., Hitachi, Japan

Nano Biomedicine 2011;3(2): 261-266, (Dec 30)
Synopsis
Human mesenchymal stem cells (hMSCs) were cultured in a chondrogenic differentiaton medium on a single multichannel nanopillar sheet with varying morphological parameters such as pillar diameter, pillar diameter to pitch ratio, and ditch width which limits the continuous pillar area, etc. The hMSCs were able to form spheroids on every type of nanopillar surface and their diameters were dependent on the parameters. Diameters of hMSC spheroids had a maximum value at a pillar diameter of 5 mm when the ratio of pillar diameter to pitch was fixed to 1/2 and decreased with increasing ditch width. These phenomena might be explained by the adhering area and migration speed of the hMSCs on the pillar surface.

Key words: nanopillar sheet, cell culture, mesenchymal stem cells (MSCs), chondrogenic differentiation <

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