Nano Biomedicine
ORIGINAL ARTICLE
 Study of In Vitro Embryotoxicity Potential by Two Type Nano Titanium Dioxide

 Koichi IMAI1, Tsukasa AKASAKA2, Fumio WATARI2, Akito TANOUE3, Kazuaki NAKAMURA3, Kazuhiko SUESE4,
Hiromasa TAKASHIMA5, Tetsunari NISHIKAWA6, Akio TANAKA6, and Shoji TAKEDA1

1Department of Biomaterials, Osaka Dental University, Osaka, Japan
2Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan
3Department of Pharmacology, National Research Institute for Child Health and Development, Tokyo, Japan
4Osaka Dental University. School of Dental Technician and Hygienist, Osaka, Japan
5Hatano Research Institute, FDSC, Kanagawa, Japan
6Department of Oral Pathology, Osaka Dental University, Osaka, Japan

Nano Biomedicine 2011;3(1): 224-230, (Jun 30)
Synopsis
Nano and submicron titanium dioxides are widely used as ingredients of sun screen. Thus, its re-productive and developmental toxicities in humans are of concern. We used two kinds of nano titanium dioxides, involving different production methods and surface coatings. The embryotoxicities of these nano titanium dioxides were determined based on inhibitory effects on the cardiomyocyte differentiation of mouse Embryonic Stem cells. As ultrafine titanium dioxide, titanium dioxide (particle diameter, 0.03-0.05 µm) produced by the sintering method (T1) and that (particle diameter, 0.01-0.02 µm) produced by the wet method (T2) were used. T2 may cause little developmental toxicity because it has no potent inhibitory effects on cardiomyocyte differentiation. However, T1 should be further investigated.

Key words: titanium dioxide, ES cells, EST<

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