Synopsis
To examine the effects of C60 fullerene on angiogenesis, we investigated the effects of C60 fullerene on capillary regeneration from cells in the early stage of tubule-like structures using a human angiogenesis kit (Kurabo, Tokyo). In addition, using 8-Hydroxydeoxyguanosine (8-OHdG), we investigated its effects as an antioxidant to inhibit oxidative stress. The area ration and length of new blood vessels in the 5mg/mL group were 57.4 and 54.3%, respectively, compared with the control group. At 2.5 and 5 mg/mL, no significant difference was noted compared with the control group. The amounts of 8-OHdG in the 2.5 and 5 mg/mL groups were 89.4 and 87.5%, respectively, on comparison with the control group. Thus, no significant difference was noted. C60 fullerene was not sufficiently dispersed in the medium. Thus, C60 fullerene particles should be homogeneously dispersed in media. In the future, our results should be compared with those from dissolution using Polyvinylpyrrolidone (PVP) to clarify biological evaluation of new capillary formation.
Key words: fullerene, C60, angiogenesis, LDH, 8-OHdG
