Nano Biomedicine
ORIGINAL ARTICLE
 Culture of ES Cells and Mesenchymal Stem Cells on Carbon Nanotube Scaffolds

 Hiromi KITAHARA1, Yoshinori KUBOKI2, Hiroko TAKITA3, Tsukasa AKASAKA2, Fumio WATARI2, and Nobuo INOUE1

1Department of Dentistry for a Senior Person,
2Department of Biomedical, Dental Materials and Engineering,
3Support Section for Education and Research,
Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan

Nano Biomedicine 2010;2(2): 81-92, (Dec 30)
Synopsis
Cell proliferations on carbon nanotube scaffolds were investigated for the embryonic stem cells (ES cells) of mouse and the bone marrow-derived mesenchymal stem cells (MSC) of rat. Although ES cells were well attached and proliferated on gelatin-coated plastic dishes, they did not attach on the CNT-coated scaffold, and formed the spheroids growing in the culture medium. For MSC, they attached on the CNT-coated scaffold and grew to form mineralized particles as on the plastic dishes of control. The growth rate of the MSC was lower than control but showed the higher expression of alkaline phosphatase activity per cell in 2 weeks and degree of mineralization per cell for 3 and 4 weeks.

Key words: carbon nanotubes, ES cell, mesenchymal stem cells, ALP activity, mineralization

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