Nano Biomedicine
Influences of In Vitro Tubule-like Structures by Two Types of Ultrafine Titanium Dioxide and Zinc Oxide

Koichi IMAI1, Tetsunari NISHIKAWA2, Akio TANAKA2, Fumio WATARI3, Hiromasa TAKASHIMA4, and Shoji TAKEDA1

1Department of Biomaterials, Osaka Dental University, Osaka, Japan
2Department of Oral Pathology, Osaka Dental University, Osaka, Japan
3Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan
4Hatano Research Institute, FDSC, Kanagawa, Japan

Nano Biomedicine 2010;2(1): 52-58, (Jun 30)

We evaluated the in vitro influences of two types of ultrafine titanium dioxide differing in surface treatment and ultrafine zinc oxide on the area rate and length of tubule-like structures using a human angiogenesis kit. In addition, the influences of titanium and zinc oxide ions were evaluated. A comparison of influences on tubule-like structures between the two surface treatment methods of ultrafine titanium dioxide showed only slight influences of surface treatment providing water-repellency. Using ultrafine zinc oxide, no tubule-like structure formation was observed. The area rate of tubule-like structures was 84.0% for titanium ions and 78.3% for zinc ions at a concentration of 2.5 ppm, but this rapidly decreased with an increase in the ion concentration. These results may differ from those obtained in the nanoparticle dispersion state. It is possible that in vivo biological influences also differ between aggregation and dispersion states. Further studies based on the in vivo dispersion state are necessary.

Key words: titanium dioxide, zinc oxide, ultrafine, tubule-like structure, human angiogenesis kit

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