We examined the influence of culture methods with mono- and multilayer nanocarbon tubes (SWCNT and MWCNT, respectively) on the differentiation of mouse embryonic stem (ES) cells using a cellular differentiation technique for the embryonic stem cell test (EST). On differentiation assay, beating was noted in 72, 35, and 60% of the cells in the control, SWCNT, and MWCNT groups, respectively. In addition, there was no influence on cytotoxicity based on the LDH level. In this study, the MWCNT did not influence the differentiation of ES cells, but the SWCNT markedly inhibited it, confirming that the differentiation of ES cells depends on the material surface structure.
Key words: nanocarbon tube, embryotoxicity, ES cell, SWCNT, MWCNT