Nano Biomedicine
In Vitro Study of Cell Differentiation by Mouse Embryo Stem Cells on Nanocarbon Tubes

Koichi IMAI1, Kazuhiko SUESE2, Tsukasa AKASAKA3, Fumio WATARI3, and Shoji TAKEDA1

1Department of Biomaterials, Osaka Dental University, Osaka, Japan
2Osaka Dental University. School of Dental Technician and Hygienist, Osaka, Japan
3Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan

Nano Biomedicine 2010;2(1): 47-51, (Jun 30)

We examined the influence of culture methods with mono- and multilayer nanocarbon tubes (SWCNT and MWCNT, respectively) on the differentiation of mouse embryonic stem (ES) cells using a cellular differentiation technique for the embryonic stem cell test (EST). On differentiation assay, beating was noted in 72, 35, and 60% of the cells in the control, SWCNT, and MWCNT groups, respectively. In addition, there was no influence on cytotoxicity based on the LDH level. In this study, the MWCNT did not influence the differentiation of ES cells, but the SWCNT markedly inhibited it, confirming that the differentiation of ES cells depends on the material surface structure.

Key words: nanocarbon tube, embryotoxicity, ES cell, SWCNT, MWCNT

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