Nano Biomedicine
Synthesis of Hydroxyapatite Contining Manganese and Its Evaluation of Biocompatibility

Wataru FUJITANI1, Yoshinosuke HAMADA2, 3, Naomasa KAWAGUCHI2, Seiji MORI2, Kiyoshi DAITO3, Ayako UCHINAKA2, Takuya MATSUMOTO4, Yoshihisa KOJIMA2, Michiharu DAITO3, Takayoshi NAKANO1, and Nariaki MATSUURA2

1Department of Materials Science and Engineering, Graduate School of Engineering, Osaka University, Osaka, Japan
2Department of Molecular Pathology, Graduate School of Medicine and Health Science, Osaka University, Osaka, Japan
3Department of Pediatric Dentistry, Osaka Dental University, Osaka, Japan
4Department of Oromaxillofacial Regeneration, Graduate School of Dentistry, Osaka University, Osaka, Japan

Nano Biomedicine 2010;2(1): 37-46, (Jun 30)

Biomaterials made using hydroxyapatite (HAp) and calcium phosphates, which are inorganic components of hard tissues, have been developed to fill bone defects caused by various diseases. A biomaterial of HAp containing collagen, an organic component of bone, has also been developed. However, the physical and biological functions of these bone biomaterials remain inadequate.
Focusing on Zinc(Zn) and Manganese(Mn) ions out of the divalent cations involved in the activity of integrins, which are molecules existing in cell membranes, we synthesized three kinds of inorganic biomaterials?pure HAp, Zn-containing HAp (Zn-HAp), and Mn-containing HAp (Mn-HAp)-by a wet process. These three kinds of HAp powders were formed into pellets of 6mm diameter and 1mm thickness and their bio-adaptability was investigated.
In vitro experiments using osteoblast-like cells (MC3T3E1) demonstrated that Mn-HAp showed higher cell adhesion potential than pure HAp, indicating that Mn addition improves bioresponses. This may be because Mn ions eluted from Mn-HAp pellets activated integrins, which are closely related to the cell adhesion potential of MC3T3E1 cells to the surface of the pellets.

Key words: hydroxyapatite(HAp), manganese, biocompatibility

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