Nano Biomedicine
ORIGINAL ARTICLE

Comparison of Cell Viability Between Mouse Liver-derived NCTC Clone 1469 Cells and Balb/c 3T3 Cells Exposed to Seven Metal Ions

Tsubasa SHIRAI1, Tetsuya HIRATA1, Sayaka KAWABATA1, Xuanfeng LI1, Naomi EGGEN2, Kumiko KAJI3, Koichi IMAI4, and Yoshiya HASHIMOTO1


1Department of Biomaterials, School of Dentistry, 2Graduate School of Health Sciences,
3Department of Oral Health Sciences, Faculty of Health Sciences,
4Department of Tissue Engineering, School of Dentistry,
Osaka Dental University, Osaka, Japan
Nano Biomed 2025; 17(2): 101-106, (Dec 30, Nano Biomedicine)
Synopsis
Various cultured cell lines have been employed in cytotoxicity assays that evaluate the safety of chemical substances for human use. ISO 10993-5 and domestic medical device guidance documents both stipulate the use of mouse-derived L929 cells, Balb/c 3T3 clone A31 cells, and V79 cells as standard cell lines. Numerous other cultured cells have also been selected in various cytotoxicity studies. However, the extent to which test results vary when using alternative cultured cells has not been sufficiently investigated. In the present study, we compared the cell viability of NCTC clone 1469 cells, mouse liver–derived cells exhibiting stable proliferation, with that of standard Balb/c 3T3 clone A31 cells following exposure to seven metal ions: Ag, Au, Cu, Pd, Sn, Ti, and Zr. The results obtained showed that changes in cell viability following exposure to most metal ions were generally similar between the two cell lines. These results suggest that the use of cultured cells derived from various organs, in addition to the standard cell lines widely employed in cytotoxicity testing, provides valuable information for elucidating the mechanisms of action of various chemical substances, including materials used in regenerative medicine.

Key words: NCTC clone 1469 cells, Balb/c 3T3 cells, metal ions, cytotoxicity, MTT
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DOI: 10.11344/nano.17.101

Shirai T, Hirata T, Kawabata S, Li X, Eggen N, Kaji K, Imai K. Comparison of cell viability between mouse liver-derived NCTC clone 1469 cells and Balb/c 3T3 cells exposed to seven metal ions. Nano Biomed 2025; 17: 101-106.