Nano Biomedicine
ORIGINAL ARTICLE

Effects of vitamin B6 on Dexamethasone-induced Mineralized Nodule Formation by Rat Mesenchymal Stem Cells

Ayano MIYAMOTO, Hitomi NAKAMA, Masayuki ZENNYU, Hiroshi MAEDA, and Masataka YOSHIKAWA


Department of Endodontics, School of Dentistry,
Osaka Dental University, Osaka, Japan


Nano Biomed 2025; 17(2): 91-100, (Dec 30, Nano Biomedicine)
Synopsis
Mesenchymal stem cells (MSCs) are essential for the regeneration of dentine or alveolar bone. Although cells obtained from rat femoral bone marrow (rBMCs) have generally been used for odontogenesis, MSCs in dental pulp cells (DPCs) may be a superior option. However, the number of MSCs in dental pulp tissue is extremely low. A long period is required for MSCs in DPCs to differentiate into odontoblasts. Dexamethasone (Dex) induces the differentiation of MSCs into blast cells. We herein propose use of a novel chemical substance to replace or act as a co-factor for Dex to realize more prompt differentiation of MSCs, and vitamin B6 (VB6) was examined as one. rBMCs were used in the present study. The effects of VB6 on the mineralized nodule formation were estimated by measuring Ca2+ levels. It was shown that Dex is an essential factor for MSCs to differentiate and mineralized nodule formation, and also that VB6 acts as a co-factor for Dex.

Key words: Vitamin B6, Dexamethasone, Mineralized nodule, in vitro, Mesenchymal stem cells
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DOI: 10.11344/nano.17.91

Miyamoto A, Nakama H, Zennyu M, Maeda H, Yoshikawa M. Effects of vitamin B6 on dexamethasone-induced mineralized nodule formation by rat mesenchymal stem cells. Nano Biomed 2025; 17: 91-100.