Nano Biomedicine
ORIGINAL ARTICLE

Cytotoxic Effect of Ultrasmall Platinum Nanoparticles on RAW264 Cells

Kazuko YAMAMOTO1, Kengo IWASAKI2, Akiko SHIMADA3,
and Masanori HASHIMOTO4


1Doctor's Course of Oral Health Sciences, Graduate School of Health Sciences,
2Division of Creative and Integrated Medicine, Advanced Medical Research Center,
Translational Research Institute for Medical Innovation (TRIMI),
3Department of Oral Health Sciences, Faculty of Health Sciences,
4Department of Oral Health Engineering, Faculty of Health Sciences,
Osaka Dental University, Osaka, Japan
Nano Biomed 2025; 17(2): 49-56, (Dec 30, Nano Biomedicine)
Synopsis
Effects of ultrasmall platinum nanoparticles (PtNPs) on RAW264 cells were examined using cell viability assays, flow cytometry, and transmission electron microscopy (TEM). RAW 264 cells were exposed to the three concentrations of PtNPs with particle sizes of 4.4 nm (ultrasmall NPs) and 31.0 nm (large NPs). Cytotoxicity assays revealed no significant NP size-dependent differences in cell viability; however, both particle types caused a pronounced reduction in viability at higher concentrations compared with untreated controls. TEM imaging showed receptor-mediated endocytosis for both ultrasmall and large PtNPs. Although exposure to PtNPs clearly reduced cell viability relative to the controls, distinguishing size-specific effects remains challenging. Further comprehensive studies are required to elucidate the various cellular responses toward ultrasmall PtNPs and their potential size-dependent mechanisms of action.

Key words Ultrasmall, platinum nanoparticle, cell viability, FACS, TEM
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DOI: 10.11344/nano.17.49

Yomamoto K, Iwasaki K, Shimada A, Hahimoto M. Cytotoxic effect of ultrasmall platinum nanoparticles on RAW264 cells. Nano Biomed 2025; 17: 49-56.