Nano Biomedicine
ORIGINAL ARTICLE

Inhibitory Effect of Oral Care Tablets Containing Three Powdered Phytochemicals on the Proliferation of Porphyromonas Gingivalis

Tomoharu OKAMURA1, Masumi MORI2, Chikako YAMAOKA2, Saki KAWAHARA2, Saki KOSHINO3, Katsuaki SUZUKI1, Suguru DATEOKA1, Aki NISHIURA2, Yoshiaki ONO3, and Kazuya TOMINAGA1


1Department of Oral Pathology, 2Department of Orthodontics, 3Department of Special Care Dentistry,
School of Dentisty, Osaka Dental University, Osaka, Japan
Nano Biomed 2025; 17(1): 9-13, (Jun 30, Nano Biomedicine)
Synopsis
We have reported that oral care tablets (Rifrele) containing three types of phytochemicals inhibit the growth of early colonizing bacteria (cariogenic bacteria) that form dental plaque in vitro. In this study, we investigated whether Rifrele affects the growth of Porphyromonas gingivalis (P. g.) in vitro. Four different concentrations of Rifrele were added to P. g. that had been anaerobically cultured at 37°C for one week. As a result, the number of colonies formed 30 minutes after addition changed. The viable bacterial count increased in the group without Rifrele and in the group with Rifrele added at 25 mg/mL. In contrast, the viable bacterial count decreased in the groups with Rifrele added at 50 mg/mL, 100 mg/mL, and 200 mg/mL. The viable bacterial count was lowest in the group with Rifrele added at 200 mg/mL. The reduction rate of viable cell count was highest in the group where Rifrele was added at 200 mg/mL. It was shown that Rifrele affects the growth of P. g., and it was suggested that high concentrations of Rifrele suppress the growth of P. g. after 30 minutes of incubation.

Key words:phytochemicals, Porphyromonas gingivalis, inhibit growth