Nano Biomedicine
ORIGINAL ARTICLE

Effects of Vitamin B12 on Mineralized Nodule Deposition by Dental Pulp Cells from Immunosuppressant-treated Rats

Hitomi NAKAMA, Masataka YOSHIKAWA, Hideaki IKENAGA, Norimasa TSUJI, Ayano MIYAMOTO, Motoki USHIKUBO, and Hiroshi MAEDA

 Department of Endodontics, School of Dentistry,
Osaka Dental University, Osaka, Japan
Nano Biomed 2024; 16(2): 79-88, (Dec 30, Nano Biomedicine)
Synopsis
Bone marrow cells from the rat femur, which contain mesenchymal stem cells (MSCs), have frequently been used for bone and tooth regeneration both in vivo and in vitro. However, since a low number of MSCs are present in dental pulp cells (DPCs), hard tissue regeneration in vitro requires a long period of time. Therefore, bioactive substances are needed to promote the proliferation and differentiation of these cells. DPCs from rats (rDPCs) treated with a subcutaneous injection of an immunosuppressant formed significantly more mineralized nodule aggregates in subcultures with dexamethasone (Dex). The effects of vitamin B12 (VB12) on the formation of mineralized nodules in subcultures of rDPCs have also been investigated. VB12 was confirmed to be effective for the pro-liferation and differentiation of MSCs in a subculture with Dex. Therefore, we herein investigated the effects of VB12 on MSC proliferation, differentiation, and osteogenesis in DPCs from immuno-suppressant-injected rats. VB12 effectively enhanced the formation of mineralized nodule aggre-gates in a subculture of rDPCs.

Key words: mineralized nodules, dental pulp cells, dexamethasone, immunosuppressant, vitamin B12
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DOI: 10.11344/nano.16.79

Nakama H, Yoshikawa M, Ikenaga H, Tsuji N, Miyamoto A, Ushikubo M, Maeda H. Effects of vitamin B12 on mineralized nodule deposition by dental pulp cells from immunosuppressant-treated rats. Nano Biomed 2024; 16(2): 79-88.