Nano Biomedicine
ORIGINAL ARTICLE

Sealing Property of Dentin Tubules by A New Hypersensitivity Control Material and Its Effect on Human Cultured Cells

Tomoharu OKAMURA1, Harumi ISONO1, Katsuhiro SUZUKI1,
Yukino HISANO1, Takehiro YOSHIKANE1, Chihoko IKEDA1, Aya INOUE2,
Daisuke OKAMORI2, Sho AOKI3, Takeshi INAMOTO4, Tetsunari NISHIKAWA5,
Hiroshi MAEDA4, and Kazuya TOMINAGA4

 1 Department of Oral Pathology, 2 Department of Orthodontics, 3 Department of Pediatric Dentistry,
4 Department of Endodontics, 5 Department of Innovations in Dental Education,
Osaka Dental University, Osaka, Japan
Nano Biomed 2024; 16(1): 42-50, (Jun 30, Nano Biomedicine)
Synopsis
This study was conducted to observe the dentin tubule sealing and biocompatibility of a newly developed dental hypersensitivity control material. The Fourth Dentin® (4D) made mainly from nano-sized calcium carbonate was applied to bovine anterior dentin and observed vertically using a scanning electron microscope. Because 4D forms film on dentin and 4D vertically seals dentin tubules, human periodontal ligament fibroblasts, human deciduous dental pulp-derived fibroblasts, and human osteoblasts were cultured with medium-added 4D. As a result, the cell proliferative ability of each cell to which 4D was added was enhanced. Each cell was also reacted with 4D and the condition of the cells around 4D was observed. The results showed that 4D enhanced mitochondrial activity in human periodontal ligament fibroblasts, human deciduous dental pulp fibroblasts, and human osteoblasts, and that each of these cells proliferated around 4D, suggesting a biocompatibility between these cells and 4D.

Key words: dentin hypersensitivity, calcium carbonate, fibroblast, osteoblast, biocompatibility


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DOI : "https://doi.org/10.11344/nano.16.42"

J-stage : Okamura T, Isono H, Suzuki K, Hisano Y, Yoshikane T, Ikeda C, Inoue A, Okamori D, Aoki S, Inamoto T, Nishikawa T, Maeda H, Tominaga K. Sealing property of dentin tubules by a new hypersensitivity control material and its effect on human cultured cells. Nano Biomed 2024; 16: 42-50.