Nano Biomedicine
ORIGINAL ARTICLE

Cytotoxicity of Ultrasmall Silver Nanoparticles to RAW264 Cells

Shiki HO1, Masanori HASHIMOTO2, Takahiro SHUTO1,
Kazuko YAMAMOTO2, and Kengo IWASAKI3
 1Department of Oral Health Engineering, 2Department of Oral Health Science, Faculty of Health Sciences,
3Division of Creative and Integrated Medicine, Advanced Medical Research Center,
Translational Research Institute for Medical Innovation (TRIMI),
Osaka Dental University, Osaka, Japan
Nano Biomed 2024; 16(1): 35-41, (Jun 30, Nano Biomedicine)
Synopsis
Nanoparticles (NPs) exhibit different functions to larger particles because of their specific surface areas. Ultrasmall NPs (particle size < 10 nm) could exhibit novel functions because they are even smaller. This study aimed to evaluate the effect of ultrasmall silver NPs on RAW264 cells using cell viability assay, scanning electron microscopy, and transmission electron microscopy. Silver NPs with particle sizes of 8.2 and 34.8 nm were evaluated, and cells were exposed to these NPs at three different concentrations (25, 50, or 100 μg/mL). A clear decrease in cell viability compared with the control group was observed with 8.2 nm NPs at 100 μg/mL. The cell viability did not decrease in the other groups. No morphological differences were observed among the groups by scanning electron microscopy. In transmission electron microscopy, various morphological change were observed for the cells exposed to NPs. Further research using different biological assays is required to elucidate the effect of the ultrasmall NPs at a high concentration on cell viability.

Key words: Ultrasmall nanoparticle, RAW264 cell, Cell viability, SEM, TEM

All documents
DOI : "https://doi.org/10.11344/nano.16.35"

J-stage : Ho S, Hashimotp M, Shuto T, Yamamoto K, Iwasaki K. Cytotoxicity of ultrasmall silver manoparticles to RAW264 cells. Nano Biomed 2024; 16: 35-41.