Nano Biomedicine
ORIGINAL ARTICLE

Capillary Stem Cells Enhance Vascular Barrier Function Revealed by the In Vitro Microvessel Model

Hedele ZENG1, 2, Takanori SANO1, Jean CACHEUX1, 3
Aurélien BANCAUD1, 3, Jun-ichi KAWABE4,
and Yukiko T. MATSUNAGA1, 2, 3

 1Institute of Industrial Science, The University of Tokyo, Tokyo, Japan
2Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan
3LIMMS, CNRS-IIS UMI 2820, The University of Tokyo, Tokyo, Japan
5Department of Biochemistry, Asahikawa Medical University, Asahikawa, Japan
Nano Biomed 2023; 15(2):105-111 (Dec 30, Nano Biomedicine)
Synopsis
Mesenchymal stem cells (MSCs) have received attention in the field of regenerative medicine due to their broad-ranging differentiation potential and strong secretory function. Although some studies have shown that MSC transplantation rescues blood flow, its contribution to the repair of microvasculature at the cell-to-tissue level is still obscure. To understand the mechanism of the MSC therapy for ischemic diseases, we developed a three-dimensional (3D) microvessel model cocultured with EphA7+ MSC-like capillary derived pericytes, termed capillary stem cells (CapSCs). It was shown that cocultured CapSCs within the collagen gel matrix tend to accumulate around the microvessel, accompanied with junctional expression VE-cadherin on the endothelial layer. In addition, the barrier function assay showed CapSCs strengthen the endothelial barrier.

Key words: Mesenchymal stem cells (MSCs), capillary stem cells (CapSCs), capillary endothelial barrier, permeability assay, in vitro 3D model


All documents
DOI : "https://doi.org/10.11344/nano.15.105"

J-stage : Zeng H, Sano T, Cacheux J, Bancaud A, Kawabe J, Matsunaga YT. Capillary stem cells enhance vascular barrier function revealed by the In vitro microvessel model. Nano Biomed 2023; 15: 105-111.