Synopsis
The present study attempted to obtain factors that induce the calcified nodule formation in a mesenchymal stem cell (MSC) culture through the aggregation of micro- or nanoparticles, such as calcium carbonate. There are few suitable cell sources in the oral cavity other than dental pulp tissue, from which only a small number of MSCs may be obtained. However, a large number of MSCs are needed for tooth regeneration. Therefore, factors that promote MSC proliferation and differentiation are required. Vitamin B₁ (VB₁), vitamin B₃ (VB₃) and vitamin B₅ (VB₅), which are present in physiologically active substances, were selected and investigated their effects to bone formation in vitro. VB₁ and VB₃ significantly increased the ability of dexamethasone (Dex) to form calcified nodule aggregates in vitro, whereas VB₅ exerted the opposite effects. Therefore, it was confirmed from the results of this in vitro study that VB₁ and VB₃ may affect to Dex to promote MSCs in rat bone marrow cells for proliferation and differentiation into hard tissue-forming cells and the formation of calcified nodule aggregates.

Key words: Vitamin B, Calcified nodules, Bone marrow cells, Dexamethasone

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DOI : "https://doi.org/10.11344/nano.15.51"

J-stage : Nakama H, Yoshikawa M, Matsuo N, Maeda H. Effects of vitamins added to culture medium for calcified nodule formation by rat bone marrow cells. Nano Biomed 2023; 15: 51-65.