Nano Biomedicine
ORIGINAL ARTICLE

Muramyl Dipeptide (MDP) Regulates the Production of Matrix Metalloproteinase-3 by Human Dental Pulp Fibroblast-like Cells

Yang YAN1, Hiroshi INOUE1, Hidetoshi MOROTO2,
Kazuyo YAMAMOTO2, and Seiji GODA1

 1Department of Physiology, 2Department of Operative Dentistry, School of Dentistry,
Osaka Dental University, Osaka, Japan
Nano Biomed 2023; 15(1):14-20, (Jun 30, Nano Biomedicine)
Synopsis
Nucleotide-binding oligomerization domain 2, which is constitutively expressed in human dental pulp fibroblast-like cells (hDPFs), plays a role in pulpal immune responses by sensing muramyl dipeptide (MDP) from bacteria. Matrix metalloproteinase-3 (MMP-3) is abundantly expressed in acute pulpitis and acts as a mediator of the healing of dental pulp as an anti-inflammatory and regenerative factor. In the present study, we investigated the involvement of the ERK1/2 signaling pathway in MMP-3 production by MDP-stimulated hDPFs. MDP was not cytotoxic against hDPFs and did not affect their proliferation. A stimulation with MDP enhanced MMP-3 production and ERK1/2 phosphorylation. The ERK1/2 signaling pathway was involved in the production of MMP-3 by MDP-stimulated hDPFs. These results suggest that the induction of MMP-3 production by MDP-stimulated hDPFs has an anti-inflammatory function by degrading inflammatory cytokines.

Key words: MDP, MMP-3, ERK1/2, human dental pulp fibroblast-like cells

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DOI "https://doi.org/10.11344/nano.15.14"

J-stage Yan Y, Inoue H, Morito H, Yamamoto K, Goda S. Muramyl dipeptide (MDP) regulates the production of matrix metalloproteinase-3 by human dental pulp fibroblast-like cells. Nano Biomed 2023; 15(1): 14-20.