Nano Biomedicine
ORIGINAL ARTICLE

γ-D-glutamyl-meso-diaminopimelic Acid (iE-DAP) Regulates the Production of Matrix Metalloproteinase-1 by Human Dental Pulp Fibroblast-like Cells

Zhengjian KE1, Hiroshi INOUE1, Shinji MATSUO2,
Kousuke KASHIWAGI2, and Seiji GODA1

 1Department of Physiology, 2Department of Fixed Prosthodontics and Occlusion,
School of Dentistry, Osaka Dental University, Osaka, Japan
Nano Biomed 2023; 15(1):7-13, (Jun 30, Nano Biomedicine)
Synopsis
Nucleotide-binding oligomerization domain 1, which is constitutively expressed in human dental pulp fibroblast-like cells (hDPFs), plays a role in pulpal immune responses by sensing γ-D-glutamyl-meso-diaminopimelic acid (iE-DAP) from bacteria. Matrix metalloproteinase-1 (MMP-1) is an important enzyme that degrades type I collagen, an extracellular matrix component, and is involved in the destruction of dental pulp tissue. In the present study, we investigated the involvement of the ERK1/2 signaling pathway in MMP-1 production by iE-DAP-stimulated hDPFs. iE-DAP was not cytotoxic against hDPFs and did not affect their proliferation. A stimulation with iE-DAP enhanced MMP-1 production and ERK1/2 phosphorylation. The ERK1/2 signaling pathway was involved in the production of MMP-1 by iE-DAP-stimulated hDPFs. These results suggest that the induction of MMP-1 production by iE-DAP-stimulated hDPFs exacerbates pulpitis by promoting the degradation of surrounding collagen.

Key words: iE-DAP, MMP-1, ERK1/2, human dental pulp fibroblast-like cells

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DOI "https://doi.org/10.11344/nano.15.7"

J-stage Ke Z, Inoue H, Matsuo S, Kawasaki K, Goda S. γ-D-glutamyl-meso-diaminopimelic acid (iE-DAP) regulates the production of matrix metalloproteinase-1 by human dental pulp fibroblast-like cells. Nano Biomed 2023; 15(1): 7-13.