Nano Biomedicine
ORIGINAL ARTICLE

Comparison of Cell Viability Between ES-D3 Cells and Balb/c 3T3 Cells by Pressurized Cell Culture using a Centrifugal Incubator

Koichi IMAI1, 2, Masahiro MASUDA3, Junyi WANG3, Cheng TAN3, Mamoru UEDA3, Koichiro JIN2, Kumiko KAJI2, Reiko ASHIDA2, Mihoko YONEZAWA2, and Yoshiya HASHIMOTO3

 1Department of Tissue Engineering, 2Faculty of Health Sciences, 3Department of Biomaterials,
Osaka Dental University, Osaka, Japan
Nano Biomed 2022; 14(2):67-72, (Dec 30, Nano Biomedicine)
Synopsis
The in vivo environment is a state in which cells are always present under pressures, such as blood pressure. Previous studies examined the influence of pressures, such as gravity, on biotissue, that is, various effects on tissues and cells. We prepared a specific centrifuge incubator that makes it possible to maintain the intracentrifuge temperature at 37&dgree;C from a circulation-type constant temperature water bath, and investigated its influence on the viability of mouse-derived ES and connectivetissue-derived Balb/c 3T3 (3T3) cells. The results showed that ES-D3 cell viability was lower than 3T3 cell viability when a centrifugal force was applied in the initial phase of culture. In the future, the influence on cell differentiation in addition to cell proliferation should be examined.

Key words: pressurized culture, centrifugal incubator, ES cell, Balb/c 3T3 cell, cell viability

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DOI "https://doi.org/10.11344/nano.14.67"

J-stage "Imai K, Masuda M. Wang J, Tan C, Ueda M, Jin K, Kaji K, Ashida R, Yonezawa M, Hashimoto Y. Comparison of cell viability between ES-D3 cells and Balb/c 3T3 cells by pressurized cell culture using a centrifugal incubator. Nano Biomed 2022; 14(2): 67-72."