Nano Biomedicine
ORIGINAL ARTICLE

Fabrication and Cell Behavior Assessment of Antibacterial Micro/nano-patterned Chitosan Films

Erika NISHIDA1, Hirofumi MIYAJI1, Tsutomu SUGAYA1, and Tsukasa AKASAKA2

 1Department of Periodontology and Endodontology, Faculty of Dental Medicine,
Hokkaido University, Sapporo, Japan
2Department of Biomaterials and Bioengineering, Faculty of Dental Medicine,
Hokkaido University, Sapporo, Japan
Nano Biomed 2022; 14(1):1-8, (Jun 30, Nano Biomedicine)
Synopsis
The surface of biomaterials with a fine micro/nano-pattern reportedly affects cell behavior. Chitosan was previously shown to exhibit antibacterial properties and thus here we evaluated the cellular response and antibacterial activity of chitosan micro/nano patterns prepared by the imprint method. We fabricated micro/nano patterns with different sizes and shapes of pillars, grooves, and holes. The chitosan pattern films exhibited antibacterial activity towards Streptococcus mutans. In addition, we observed mammalian cells adhering to and spreading on the chitosan pattern, and found that the orientation of the cells was controlled by the shape of the pattern.

Key words: biomaterial surfaces, chitosan, Streptococcus mutans, MC3T3-E1 cell