Nano Biomedicine
ORIGINAL ARTICLE
Comparison of Cell Viability of ES-D3 Cells with and without Addition of Mouse Leukemia Inhibitory Factor (mLIF) to Assay Medium under C60 Fullerene Exposure
Tsubasa SHIRAI1, Yoshiya HASHIMOTO1, Reiko ASHIDA3, Masaharu MOTONE3, and Koichi IMAI2,3
1Department of Biomaterials, 2Department Tissue Engineering,
3Faculty of Health Sciences, Osaka Dental University, Osaka, Japan
Nano Biomed 2021; 13(2):69-73, (Dec 30, Nano Biomedicine)
Synopsis
The spontaneous differentiation of mouse-derived ES cells is suppressed by the supplementation of mouse Leukemia Inhibitory Factor (mLIF) into the medium. ES cells immediately differentiate when cultured in mLIF-free medium. Although the effects of mLIF on ES cell differentiation are well known, its effects on cell proliferation have hardly been investigated. Furthermore, no report has been published on the effects of mLIF in medium on cell viability. C60 fullerene has potent antioxidant capacity but little toxicity in in vivo and in vitro studies. Therefore, the effects of C60 fullerene on the viability of ES-D3 cells were examined in three-dimensional culture with and without mLIF, demonstrating no significant difference due to the supplementation of mLIF. Thus, we obtained data required for examining the effects of mLIF on the differentiation of ES-D3 cells.
Key words: C60 fullerene, ES-D3 cells, mouse leukemia inhibitory factor, mLIF, cell viability
All documents in this paper (Free)
J-Stage https://www.jstage.jst.go.jp/article/nano/13/2/13_13_69/_article
DOI https://doi.org/10.11344/nano.13.69
The spontaneous differentiation of mouse-derived ES cells is suppressed by the supplementation of mouse Leukemia Inhibitory Factor (mLIF) into the medium. ES cells immediately differentiate when cultured in mLIF-free medium. Although the effects of mLIF on ES cell differentiation are well known, its effects on cell proliferation have hardly been investigated. Furthermore, no report has been published on the effects of mLIF in medium on cell viability. C60 fullerene has potent antioxidant capacity but little toxicity in in vivo and in vitro studies. Therefore, the effects of C60 fullerene on the viability of ES-D3 cells were examined in three-dimensional culture with and without mLIF, demonstrating no significant difference due to the supplementation of mLIF. Thus, we obtained data required for examining the effects of mLIF on the differentiation of ES-D3 cells.
Key words: C60 fullerene, ES-D3 cells, mouse leukemia inhibitory factor, mLIF, cell viability
All documents in this paper (Free)
J-Stage https://www.jstage.jst.go.jp/article/nano/13/2/13_13_69/_article
DOI https://doi.org/10.11344/nano.13.69