C60 (fullerene) nanomaterials have raised health concerns. In our experiment, insoluble C60 fullerene was adsorbed with cells on the surface of glass fibers without a solvent. Cell viability was examined in conventional two- and glass fiber-based three-dimensional cultures. As a result, C60 fullerene had no significant effect on the cell viability in both two- and three-dimensional cultures. No significant difference was noted between these cultures. Biosafety assays for materials, such as C60 fullerene, insoluble in culture medium, should be further investigated.
Key words: cell viability, C60 fullerene, glass fiber, three-dimensional culture, two-dimensional culture
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