Nano Biomedicine
ORIGINAL ARTICLE
Cell Viability of C60 Fullerene with Three-dimensional Culture using Glass Fiber and Two-dimensional Culture
Yasuhiko MATSUSHIMA1, and Yumi HOSHINO 2
1Matsushima Dental Clinic, Osaka, Japan
2Kanagawa Dental University Junior College, Kanagawa, Japan
Nano Biomed 2020; 12(2):110-114, (Dec 30, Nano Biomedicine)
Synopsis
C60 (fullerene) nanomaterials have raised health concerns. In our experiment, insoluble C60 fullerene was adsorbed with cells on the surface of glass fibers without a solvent. Cell viability was examined in conventional two- and glass fiber-based three-dimensional cultures. As a result, C60 fullerene had no significant effect on the cell viability in both two- and three-dimensional cultures. No significant difference was noted between these cultures. Biosafety assays for materials, such as C60 fullerene, insoluble in culture medium, should be further investigated.
Key words: cell viability, C60 fullerene, glass fiber, three-dimensional culture, two-dimensional culture
All documents in this paper (Free)
J-Stage https://www.jstage.jst.go.jp/article/nano/12/2/12_110/_article
DOI https://doi.org/10.11344/nano.12.110
C60 (fullerene) nanomaterials have raised health concerns. In our experiment, insoluble C60 fullerene was adsorbed with cells on the surface of glass fibers without a solvent. Cell viability was examined in conventional two- and glass fiber-based three-dimensional cultures. As a result, C60 fullerene had no significant effect on the cell viability in both two- and three-dimensional cultures. No significant difference was noted between these cultures. Biosafety assays for materials, such as C60 fullerene, insoluble in culture medium, should be further investigated.
Key words: cell viability, C60 fullerene, glass fiber, three-dimensional culture, two-dimensional culture
All documents in this paper (Free)
J-Stage https://www.jstage.jst.go.jp/article/nano/12/2/12_110/_article
DOI https://doi.org/10.11344/nano.12.110