Nano Biomedicine
ORIGINAL ARTICLE
Desorption and Ionization of Amino Acid Compounds Observed by Negative Ion Mode Surface-Assisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) using Titanium Oxide Nanoparticles
Tetsu YONEZAWA1, 2, Takashi ASANO1, 3
and Mai Thanh NGUYEN1
1Division of Materials Science and Engineering, Faculty of Engineering, Hokkaido University, Sapporo, Japan
2Insitute for the Promotion of Business-Regional Collaboration, Hokkaido University, Sapporo, Japan
3Criminal Investigation Laboratory, Metropolitan Police Department, Tokyo, Japan
Nano Biomed 2020; 12(2):101-109, (Dec 30, Nano Biomedicine)
Synopsis
Surface-assisted laser desorption / ionization mass spectroscopy (SALDI-MS) in negative mode of various amino acids with commercial TiO2 nanoparticles as the DI assisting material is reported. SALDI-MS gives clear spectra of these low molecular weight negative ions, such as drugs and bio-related organic compounds. With commercially available TiO2 nanoparticles, 20 common amino acids used could be clearly detected as [M-H]- molecularions. The ion intensity of [M-H]- molecular ions is well linear correlated with the gas-phase acidity but less correlation with the hydrophobicity. But the concentration of the amino acids on the TiO2 nanoparticle surface also affected the ion intensity.
Key words: surface assisted laser ionization desorption, ionization, amino acids, negative ion mode, titanium oxide
All documents in this paper (Free)
J-Stage https://www.jstage.jst.go.jp/article/nano/12/2/12_101/_article
DOI https://doi.org/10.11344/nano.12.101
Surface-assisted laser desorption / ionization mass spectroscopy (SALDI-MS) in negative mode of various amino acids with commercial TiO2 nanoparticles as the DI assisting material is reported. SALDI-MS gives clear spectra of these low molecular weight negative ions, such as drugs and bio-related organic compounds. With commercially available TiO2 nanoparticles, 20 common amino acids used could be clearly detected as [M-H]- molecularions. The ion intensity of [M-H]- molecular ions is well linear correlated with the gas-phase acidity but less correlation with the hydrophobicity. But the concentration of the amino acids on the TiO2 nanoparticle surface also affected the ion intensity.
Key words: surface assisted laser ionization desorption, ionization, amino acids, negative ion mode, titanium oxide
All documents in this paper (Free)
J-Stage https://www.jstage.jst.go.jp/article/nano/12/2/12_101/_article
DOI https://doi.org/10.11344/nano.12.101