Nano Biomedicine
ORIGINAL ARTICLE
Elucidation of the Mechanism of Bone Regeneration When a Mixture of β-TCP and Autologous Bone Granules are Transplanted
Masayasu TATSUMURA1, Kenichirou YASUI1,
Yoshiya HASHIMOTO2, and Naoyuki MATSUMOTO1
1Department of Orthodontics, Osaka Dental University, Osaka, Japan
2Department of Biomaterials, Osaka Dental University, Osaka, Japan
Nano Biomed 2020; 12(2):89-100, (Dec 30, Nano Biomedicine)
Synopsis
We examined the amount and maturity of collagen as a parameter for bone quality in the graft material transplanted into a rat skull bone defect model. Male SD rats were used to generate an experimental animal model of skull defects. The obtained autologous bone granules and β-TCP granules were mixed at a ratio of 1:1 to prepare a graft material that combines autologous bone with β-TCP granules. At 8 weeks after transplantation, the β-TCP+ autologous bone and the autologous bone groups showed BV/TV values, measured based on CT image analysis, significantly different from those in the β-TCP and control groups. The β-TCP+ autologous bone group exhibited the ability to form blood vessels into the graft material for a longer period of time after transplantation compared to that in the autologous bone group, as well as the elevated production of collagen into β-TCP granules during the bone formation process compared to that in the β-TCP group.
Key words: autologous bone graft, β-TCP granules, micro-CT, picrosirius red staining, collagen, vWF immunostaining
All documents in this paper (Free)
J-Stage https://www.jstage.jst.go.jp/article/nano/12/2/12_89/_article
DOI https://doi.org/10.11344/nano.12.89
We examined the amount and maturity of collagen as a parameter for bone quality in the graft material transplanted into a rat skull bone defect model. Male SD rats were used to generate an experimental animal model of skull defects. The obtained autologous bone granules and β-TCP granules were mixed at a ratio of 1:1 to prepare a graft material that combines autologous bone with β-TCP granules. At 8 weeks after transplantation, the β-TCP+ autologous bone and the autologous bone groups showed BV/TV values, measured based on CT image analysis, significantly different from those in the β-TCP and control groups. The β-TCP+ autologous bone group exhibited the ability to form blood vessels into the graft material for a longer period of time after transplantation compared to that in the autologous bone group, as well as the elevated production of collagen into β-TCP granules during the bone formation process compared to that in the β-TCP group.
Key words: autologous bone graft, β-TCP granules, micro-CT, picrosirius red staining, collagen, vWF immunostaining
All documents in this paper (Free)
J-Stage https://www.jstage.jst.go.jp/article/nano/12/2/12_89/_article
DOI https://doi.org/10.11344/nano.12.89