We examined the amount and maturity of collagen as a parameter for bone quality in the graft material transplanted into a rat skull bone defect model. Male SD rats were used to generate an experimental animal model of skull defects. The obtained autologous bone granules and β-TCP granules were mixed at a ratio of 1:1 to prepare a graft material that combines autologous bone with β-TCP granules. At 8 weeks after transplantation, the β-TCP+ autologous bone and the autologous bone groups showed BV/TV values, measured based on CT image analysis, significantly different from those in the β-TCP and control groups. The β-TCP+ autologous bone group exhibited the ability to form blood vessels into the graft material for a longer period of time after transplantation compared to that in the autologous bone group, as well as the elevated production of collagen into β-TCP granules during the bone formation process compared to that in the β-TCP group.
Key words: autologous bone graft, β-TCP granules, micro-CT, picrosirius red staining, collagen, vWF immunostaining
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