Nano Biomedicine
ORIGINAL ARTICLE
Difference in Cell Viability between with and without Visible Light-irradiation by Camphorquinone (CQ)-containing Medium
Tsubasa SHIRAI1, Tan CHENG1, Yuzhu SUN1, Yufan WU1,
Ye ZHANG1, Yoshiya HASHIMOTO1, Yumi HOSHINO2,
Yasuhiko MATSUSHIMA3, and Koichi IMAI4, 5
1Department of Biomaterials, School of Dentistry, Osaka Dental University, Osaka, Japan
2Kanagawa Dental University Junior College, Kanagawa, Japan
3Matsushima Dental Clinic, Osaka, Japan
4Department of Tissue Engineering, School of Dentistry, Osaka Dental University, Osaka, Japan
5School of Health Sciences, Osaka Dental University, Osaka, Japan
Nano Biomed 2020; 12(2):77-82, (Dec 30, Nano Biomedicine)
Synopsis
The intense photoirradiation-induced influence of a photosensitizer, camphorquinone (CQ), contained in light-cured composite resin applied on intraoral tissue is of concern. The difference in cell viability between groups treated with and without light-irradiated culture medium containing CQ was investigated using 3T3 cells, which were used according to the OECD phototoxicity guidelines. A large difference in cell viability was noted between the photoirradiation and non-photoirradiation groups within a concentration range of 0.3125∼2.5 mg/mL. The influence of phototoxicity was previously not considered in the dental field, but as restoration methods applying intense photoirradiation in the oral cavity, such as light-cured composite resin, are now widely used clinically, the biological influence of intense photoirradiation, even though applied only for a short time, on intraoral tissue must be considered.
Key words: cell viability, photoirradiation, camphorquinone, CQ, light-cured composite resin
All documents in this paper (Free)
J-Stage https://www.jstage.jst.go.jp/article/nano/12/2/12_77/_article
DOI https://doi.org/10.11344/nano.12.77
The intense photoirradiation-induced influence of a photosensitizer, camphorquinone (CQ), contained in light-cured composite resin applied on intraoral tissue is of concern. The difference in cell viability between groups treated with and without light-irradiated culture medium containing CQ was investigated using 3T3 cells, which were used according to the OECD phototoxicity guidelines. A large difference in cell viability was noted between the photoirradiation and non-photoirradiation groups within a concentration range of 0.3125∼2.5 mg/mL. The influence of phototoxicity was previously not considered in the dental field, but as restoration methods applying intense photoirradiation in the oral cavity, such as light-cured composite resin, are now widely used clinically, the biological influence of intense photoirradiation, even though applied only for a short time, on intraoral tissue must be considered.
Key words: cell viability, photoirradiation, camphorquinone, CQ, light-cured composite resin
All documents in this paper (Free)
J-Stage https://www.jstage.jst.go.jp/article/nano/12/2/12_77/_article
DOI https://doi.org/10.11344/nano.12.77