Nano Biomedicine
MINI REVIEW
Study of Cytotoxicity Level by Combining Nanomaterials and Chemical Substances Dissolved in Culture Medium
Tsubasa SHIRAI1, Yoshiya HASHIMOTO1,
and Koichi IMAI2, 3
1Department of Biomaterials, School of Dentistry, Osaka Dental University, Osaka, Japan
2Department of Tissue Engineering, School of Dentistry, Osaka Dental University, Osaka, Japan
3School of Health Sciences, Osaka Dental University, Osaka, Japan
Nano Biomed 2020; 12(2):120-124, (Dec 30, Nano Biomedicine)
Synopsis
products prepared with nanomaterials alone are rarely developed, and nanomaterials are likely to be added to conventional chemical substances. However, there are no data on changes in the toxicity caused by interaction between nanomaterials and dissolved chemical substances. Thus, a trace amount of nano-zinc oxide, which has no effect on cell viability, was mixed with culture medium to examine the cytotoxicity level of Bis-GMA, which is routinely used as a dental monomer. As a result, the interaction between nano-zinc oxide and Bis-GMA slightly increased the cytotoxicity of Bis-GMA. Furthermore, we were clarified that the cytotoxicity levels slightly changed in the presence of a very small amount of nano titanium dioxide or nano zinc oxide compared with those in the absence of nanomaterials, but the changes were not marked. Those finding may contribute to future studies on the cytotoxicity of nanomaterials. Some of these discoveries may contribute to future research on the combined toxicity of nanomaterials.
Key words: cell viability, cytotoxicity, Bis-GMA, nano-znc oxide
All documents in this paper (Free)
J-Stage https://www.jstage.jst.go.jp/article/nano/12/2/12_120/_article
DOI https://doi.org/10.11344/nano.12.120
products prepared with nanomaterials alone are rarely developed, and nanomaterials are likely to be added to conventional chemical substances. However, there are no data on changes in the toxicity caused by interaction between nanomaterials and dissolved chemical substances. Thus, a trace amount of nano-zinc oxide, which has no effect on cell viability, was mixed with culture medium to examine the cytotoxicity level of Bis-GMA, which is routinely used as a dental monomer. As a result, the interaction between nano-zinc oxide and Bis-GMA slightly increased the cytotoxicity of Bis-GMA. Furthermore, we were clarified that the cytotoxicity levels slightly changed in the presence of a very small amount of nano titanium dioxide or nano zinc oxide compared with those in the absence of nanomaterials, but the changes were not marked. Those finding may contribute to future studies on the cytotoxicity of nanomaterials. Some of these discoveries may contribute to future research on the combined toxicity of nanomaterials.
Key words: cell viability, cytotoxicity, Bis-GMA, nano-znc oxide
All documents in this paper (Free)
J-Stage https://www.jstage.jst.go.jp/article/nano/12/2/12_120/_article
DOI https://doi.org/10.11344/nano.12.120