Nano Biomedicine
ORIGINAL ARTICLE
Cytotoxicity of S-PRG fillers: Direct exposure of fillers and eluates from the fillers to RAW264 cells
Masanori HASHIMOTO1, Haruaki KITAGAWA2,
Yusuke TAKAHASHI3, Mikako HAYASHI3,
and Satoshi IMAZATO2
1Faculty of Health Sciences, Department of Oral Health Sciences,
Osaka Dental University, Hirakata, Osaka, Japan
2Department of Biomaterials Science, Osaka University,
Graduate School of Dentistry, Suita, Osaka, Japan
3Department of Restorative Dentistry and Endodontology, Osaka University,
Graduate School of Dentistry, Suita, Osaka, Japan
Nano Biomed 2020; 12(1):3-10, (Jun 30, Nano Biomedicine)
Synopsis
A surface pre-reacted glass-ionomer (S-PRG) fillers have been used for incorporation into a variety of dental materials. Owing to their diverse applications, it is important to evaluate their biological safety under various test conditions. This study evaluated the cytotoxic effect of exposure to various concentrations of S-PRG fillers or their eluates in RAW264 cells employing cytotoxic assays and morphological observation using a transmission electron microscope. No cytotoxic effects were found in the presence of the eluates under all concentrations tested, whereas the direct exposure of higher concentrations of S-PRG fillers to RAW264 cells showed cytotoxicity. By microscopic observations, S-PRG fillers or their depositions were found in the cells or nuclei. These findings indicated that the eluates from the S-PRG fillers exerted no influence on cytotoxicity of the RAW264 cells. Although the direct exposure of the S-PRG fillers to the cells resulted in cytotoxicity due to endocytosis, filler eluates possessed no cytotoxicity. Thus, cytotoxicity of the S-PRG fillers depends on the conditions of the exposed fillers.
Key words: S-PRG, filler; eluate, cytotoxicity, TEM
All documents in this paper (Free)
J-Stage https://www.jstage.jst.go.jp/article/nano/12/1/12_3/_article
DOI https://doi.org/10.11344/nano.12.3
A surface pre-reacted glass-ionomer (S-PRG) fillers have been used for incorporation into a variety of dental materials. Owing to their diverse applications, it is important to evaluate their biological safety under various test conditions. This study evaluated the cytotoxic effect of exposure to various concentrations of S-PRG fillers or their eluates in RAW264 cells employing cytotoxic assays and morphological observation using a transmission electron microscope. No cytotoxic effects were found in the presence of the eluates under all concentrations tested, whereas the direct exposure of higher concentrations of S-PRG fillers to RAW264 cells showed cytotoxicity. By microscopic observations, S-PRG fillers or their depositions were found in the cells or nuclei. These findings indicated that the eluates from the S-PRG fillers exerted no influence on cytotoxicity of the RAW264 cells. Although the direct exposure of the S-PRG fillers to the cells resulted in cytotoxicity due to endocytosis, filler eluates possessed no cytotoxicity. Thus, cytotoxicity of the S-PRG fillers depends on the conditions of the exposed fillers.
Key words: S-PRG, filler; eluate, cytotoxicity, TEM
All documents in this paper (Free)
J-Stage https://www.jstage.jst.go.jp/article/nano/12/1/12_3/_article
DOI https://doi.org/10.11344/nano.12.3