Nano Biomedicine

Comparison of ES-D3 Cell Differentiation by Three-dimensional Culture using Collagen and Glass Fibers by Exposure to Fine Powder of Tungsten Carbide Bar and Diamond Points

Koichi IMAI1, 4, Tsubasa SHIRAI2, Kousuke KASHIWAGI3, and Yoshiya HASHIMOTO2

1Department Tissue Engineering, 2Department of Biomaterials,
3Department of Fixed Prosthodontics and Occlusion, School of Dentistry,
4School of Health Sciences, Osaka Dental University, Osaka, Japan

Nano Biomed 2019; 11(2): 65-70, (Dec 30, Nano Biomedicine)

Rotary cutting instruments for dental restoration, such as tungsten carbide burs and diamond points, are frequently used in the oral cavity; however, minute tungsten and diamond fragments may influence biological safety. To compare 3-dimensional culture methods, pig-derived type I collagen gel and glass fibers not influenced by protein contained in collagen were exposed to debris from burs and points. The embryotoxicity of chemical substances influencing the normal birth of humans is an important index. The differentiation of ES-D3 cells, an important index, was also compared. The variation in cell differentiation on glass fibers was slightly smaller than that on collagen gel, demonstrating a slightly stronger influence. In addition, the influence of the tungsten carbide bur was slightly weaker than that of the diamond point. The influence of LDH on cells was similar. The plating of the diamond point was considered to have been eluted, thereby increasing the influence. Glass fibers may be used to construct a 3-dimensional culture environment to investigate embryotoxicity using ES-D3 cells in a long-term environmental study, which is difficult with systems using collagen gel, i.e., it may be useful for long-term measurement of the embryotoxicity of environmental chemical substances. The test method using glass fibers may be applicable to biological safety in addition to embryotoxicity.

Key words: ES cell differentiation, tungsten carbide bar, diamond point, collagen, glass fiber

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