Nano Biomedicine

Cell Viability of KF and NaF using Cell Recovery Tests between Two- and Three-dimensional Cultures using Mouse-derived ES-D3 and 3T3 Cells

Tsubasa SHIRAI1, Mari AKIYAMA1, Yoshiya HASHIMOTO1, Takamitsu YOSHIDA1, Kousuke KASHIWAGI2,
Ryo NISHIKIORI3, Takahiro SHUTO3, Tomoharu OKAMURA4, Xiaoyu HAN5, Kazuyo YAMAMOTO5, and Koichi IMAI3, 6

1Department of Biomaterials, 2Department of Fixed Prosthodontics and Occlusion,
3Faculty of Health Sciences, 4Department of Oral Pathology,
5Department of Operative Dentistry, 5Department Tissue Engineering,
Osaka Dental University, Osaka, Japan

Nano Biomed 2019; 11(1): 37-42, (June 30, Nano Biomedicine)

An in vitro test to assess the toxicity levels of chemicals based on the recovery of cell growth in a normal culture environment after transient exposure is used as a cell recovery test. This test is gen-erally conducted in a two-dimensional culture. As human tissues and organs have a complicated three-dimensional structure, cell recovery tests developed in three-dimensional tissue models may be superior to those in two-dimensional cultures.
Fluorides are commonly employed in the dental field to prevent dental caries. However, their effects on human health are attracting social attention. In the present study, KF and NaF were evaluated by comparing the results of cell recovery tests between two- and three-dimensional cultures using mouse-derived ES-D3 and 3T3 cells. As a result, ES-D3 cells exhibited more significant recovery than 3T3 cells after exposure to both chemicals in the three-dimensional culture compared with in the two-dimensional culture.

Key words: NaF, KF, fluoride, three-dimensional culture, cell recovery test

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