Nano Biomedicine

Influence of Fluoride-corroded Ti-6Al-4V Alloy on Cell differentiation and Cell Viability

Koichi IMAI1, 2, Yoshiya HASHIMOTO3, Mari AKIYAMA3, Tsubasa SHIRAI3,
Mariko NAKAI3, Tadashi YOKOYAMA3, Takamitsu YOSHIDA3, Masayuki ZENNYU3,
Kousuke KASHIWAGI4, Ryo NISHIKIORI2, Takahiro SHUTO2, Xiaoyu HAN5, and Kazuyo YAMAMOTO5

1Department Tissue Engineering, 22Faculty of Health Sciences,
3Department of Biomaterials, 4Department of Fixed Prosthodontics and Occlusion,
5Department of Operative Dentistry, Osaka Dental University, Osaka, Japan

Nano Biomed 2019; 11(1): 29-36, (June 30, Nano Biomedicine)

Dental metals have toxic effects on human development, including the high toxicity of the constituent element vanadium of the standard product ASTM F136, i.e., the medical titanium material of the Ti-6Al-4V alloy used in dental implants for its mechanical strength due to the use of a large amount of pure Ti. In addition, the corrosion of pure Ti and Ti alloy implants due to fluorides in the oral cavity is a problem. Elution of the constituent element vanadium due to corrosion may have toxic effects on development. Therefore, each element composing Ti-6Al-4V was examined in an in vitro embryotoxicity test by the Embryonic Stem Cell Test (EST) protocol. As a result, only vanadium had a risk of embryotoxicity. In addition, the Ti-6Al-4V alloy was corroded with hydrofluoric acid to examine its effects on the differentiation of mouse-derived ES and iPS cells, and was found to affect the differentiation of both cell types.

Key words: Ti-6Al-4V, corroded, hydrofluoric acid, embryotoxicity, EST

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