Nano Biomedicine

Human Dentin Coated with Silver Nanoclusters Exhibits Antibacterial Activity against Streptococcus mutans

Kanako SHITOMI1, Hirofumi MIYAJI1, Saori MIYATA1,
Erika NISHIDA1, Kayoko MAYUMI1, Tsutomu SUGAYA1,
and Hideya KAWASAKI2

1Department of Periodontology and Endodontology, Faculty of Dental Medicine,
Hokkaido University, Sapporo, Japan
2Department of Chemistry and Materials Engineering, Faculty of Chemistry,
Materials and Bioengineering, Kansai University, Osaka, Japan

Nano Biomed 2019; 11(1): 21-28, (June 30, Nano Biomedicine)

Silver nanoclusters (AgNCs) are ultrasmall in size (< 2 nm) and are expected to be an effective an-tibacterial substance to combat oral infective diseases. In the present study, we synthesized AgNCs (Ag~75) as the main component for esthetic and antibacterial application against the caries pathogen Streptococcus mutans. The results showed that AgNCs significantly reduced the turbidity and viability of S. mutans. In addition, the bactericidal effects of AgNCs were confirmed by LIVE/DEAD staining. After AgNC application to human dentin, no discoloration of dentin was observed, as compared to silver diamine fluoride application, and AgNC-treated dentin showed an inhibitory effect on colony formation by S. mutans. Therefore, AgNCs appear to be beneficial for dental therapy as an antibacterial and/or esthetic substrate.

Key words: antibacterial effect, human dentin, silver nanoclusters (AgNCs), streptococcus mutans

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