Nano Biomedicine

Effect of Heating at 37°C and Peripheral Parenteral Nutrition as a Cell Preservation Solution in Cultured Human Periodontal Ligament Fibroblasts

Tomoharu OKAMURA1, Tetsunari NISHIKAWA2, Hidenori MATSUMOTO2, Tomoki TAKEUCHI1, Chihoko IKEDA1, Suguru DATEOKA3, Yoshiaki ONO3, Kaoru Inami4, Naoyuki Matsumoto4 Shizuo HIGUCHI5, Koichi IMAI6, and Kazuya TOMINAGA1

1Department of Oral Pathology, 2Department of Innovations in Dental Education,
3Department of Special Needs Dentistry, 4Department of Orthodontics,
5Department of Oral Health Engineering, 6Department of Biomaterials,
Osaka Dental University, Osaka, Japan

Nano Biomed 2018; 10(2): 91-96, (Dec 30, Nano Biomedicine)

The necrosis of cells and the mitochondrial activity of viable cells adhering to the surface of the root of teeth luxated due to trauma can influence the success rate of dental reimplantation. Cell preservation under conditions in which the osmotic pressure, pH and nutrients of the preservative solution are close to those of body fluid and internal body temperature may lead to good results. Furthermore, since there are many pathogenic microorganisms in the oral cavity, antibacterial and antifungal agents are needed. However, in clinical practice, luxated teeth are kept in milk, and many dentists preserve them in physiological saline at 4°C or room temperature. In dental care, luxation of teeth is often accompanied by trauma to the head and the teeth will be preserved for more than one hour.
   In this study, we investigated a preservative solution containing an amino acid component and the temperature in the preservation of human periodontal ligament fibroblasts (HPLF). HPLF were cultured for 24 hours, then switched to peripheral parenteral nutrition (PPN) and preserved at 37°C. After pres-ervation for one hour, mitochondrial activity was biochemically measured using an MTT assay. The influence of antiseptics, one antimicrobial and one antifungal agent, was also examined. High levels of mitochondrial activity were seen in HPLF preserved in PPN and at 37°C. This suggests that PPN is better than physiological saline and a temperature of 37°C is better than room temperature for HPLF preservation. Antiseptics did not affect HPLF preservation.

Key words: human periodontal ligament fibroblasts, preservation, peripheral parenteral nutrition, antibiotic agent, antifungal agent

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