Nano Biomedicine
ORIGINAL ARTICLE

Desorption and Ionization of Amino Acids by Surface-Assisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) using Titanium Oxide Nanoparticles in Positive Ion Mode

Takashi ASANO1, 2 and Tetsu YONEZAWA1

 1Division of Materials Science and Engineering,
Faculty of Engineering. Hokkaido University, Sapporo, Japan
2Criminal Investigation Laboratory, Metropolitan Police Department, Tokyo, Japan
Nano Biomed 2018; 10(2): 83-90, (Dec 30, Nano Biomedicine)
Synopsis
Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) with inorganic nanoparticles as desorption/ionization (DI) assisting materials, has been reported in order to decrease the background noises including matrix ions or matrix degradation products, especially in the region of < 500 Da. In this mass region, we can detect the existence of medical material components, drugs, and bio-related organic compounds. However, the mechanism of desorp-tion / ionization of SALDI-MS is not clear. Therefore, in order to understand the correlation of desorption/ionization efficiency with molecular structures of analytes, the efficiencies of 20 common amino acids in SALDI-MS with TiO2 nanoparticles are collected in positive ion mode. SALDI-MS with TiO2 nanoparticles gives sodium ion adducted mass peaks. The ion intensity of amino acids is mainly determined by the same factors regardless of the desorption/ionization method in both MALDI and proton adducted SALDI but poorer linear correlation between the ion yield and the sodium ion affinity was found in our system.

Key words: mass spectroscopy, surface-assisted laser desorption ionization, titanium oxide, nanoparticle

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J-Stage https://www.jstage.jst.go.jp/article/nano/10/2/10_nano10_83/_article/-char/en

DOI https://doi.org/10.11344/nano.10.83