Cancer tissues strongly depend on the cancer microenvironment specifically observed around cancer, where immune cell infiltration and angiogenesis, as well as fibroblast proliferation, are induced in comparison with normal tissues. Various three-dimensional culturing methods for solid cancer cells have already been reported as in vitro
experimental methods to reflect the characteristics of cancers. The spheroid cultures, constructed with cancer cells, should more accurately reflect the biological characteristics of cancers than two-dimensional cultures. However, solid cancers applicable to spheroid culturing are still limited. Previously, we compared the results of inflammatory cytokine Interleukin-6 (IL-6) between two-dimensional culture and spheroid culture with HSC-4 cells derived from human oral squamous cell carcinoma collected from the tongue. In the present study, besides IL-6, the expression of Matrix Metalloproteinase-9 (MMP-9), which plays an important role in the infiltration and metastasis of cancer cells surrounded by ECM, was examined by real-time RT-PCR, and the sizes of spheroids were observed over time.
Key words: IL-6, MMP-9, ECM, RT-PCR, HSC-4 cells
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